WebThe comet assay is a versatile technique for detecting damage and with adjustments to the protocol can be used to quantify the presence of a wide variety of DNA altering … WebBuffer Preparation: Lysis Solution: For 1 L 2.5M NaCl 500 ml 5M NaCl (PN 75888) 0.1M EDTA 200 ml 0.5M EDTA, pH 8.0 (PN 15694) 10mM Tris-HCl, pH 10 1.2 gm/L Tris (PN …

Combining a Novel Comet Assay Procedure with Automated …

WebThe comet assay is well established for quantifying DNA damage in mammalian cells and is compatible with detection of a wide array of DNA damaging agents. In fact, it can be … Webstandard alkaline comet assay was performed using Alkaline CometAssay Control Cells (CC), treated with increasing, proprietary concentrations of etoposide, embedded in low melting agarose and immobilized on 2-, 3- and 96-well specially treated slides to promote adherence. Slides were immersed in Lysis Buffer for 30- huntsman\u0027s-cup z8

What is the minimal time for the slides to incubate in lysis buffer for ...

WebThe comet assay is a method for the quantification of DNA damage induced by chemicals. Single ... the comet assay. Lysis buffer 146.11 g NaCl (2.5 mol/L), 37.23 g EDTA (100 mmol/L), 1.21 g Tris (10 mmol/L), 7.9 g NaOH and fill up to 1 L with water. Adjust the pH to 10 with NaOH. Before using the lysis buffer, add 1% of Triton WebWe generally use a Triton X-100 based lysis buffer with 10 % DMSO, which acts as a radical scavenger, protecting the naked DNA from oxidative damage, so maybe try … WebOct 26, 2024 · Step 4A: Repair enzyme treatment in the enzyme-modified comet assay . As the lysis solution may inhibit the activity of the repair enzyme, it is ‘desirable’ to establish whether a washing step was performed between the lysis step and enzyme treatment (i.e., composition of the washing buffer, number of washings and duration). huntsman\u0027s-cup z7