WebObjective To explore if conventional protein kinase C (cPKC: PKCα and PKCβ) contributes to paraquat (PQ) -induced abnormal permeability of mouse brain microvascular endothelial cells (BMECs) via the regulation of tight junction (TJ) proteins. Methods The immortalized mouse brain endothelial cell line (bEnd.3) was used to establish a monolayer blood-brain … WebJul 5, 2024 · a, b Cell viabilities of BMECs exposed to OGD or not were analyzed by CCK-8 assays after transfection. c, d Cell migration of bEnd.3 cells exposed to OGD or not was …
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Web(180 rpm/min) at 37 C. Mouse BMECs (bEnd.3; ATCC CRL-2299, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s Modified ... The DEGs in bEnd.3 cells and APEC were ... WebMar 15, 2024 · Cell culture. Mouse BMECs (bEnd.3), which are somewhat similar to human BMECs [], were obtained from Sciencell (Shanghai, China).Between passages 3 and 7, BMECs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing a low concentration of glucose (5.5 mM) with 10% fetal bovine serum and antibiotics at 37 °C … toughest female mma fighters
EGb761 Protects Brain Microvascular Endothelial Cells …
Web(A) TEER of bEnd.3 monolayer exposed to U-87CM produced from U-87 cells in normoxic conditions (21% O2). The arrow indicates the start of monolayer treatment with U-87CM at the end of day 4. WebMice BMECs, bEnd.3 cells, were purchased from Shanghai Chunmai Biotechnology Co., Ltd and cultured in a DMEM medium containing 10% FBS at 37 ℃ in 5% CO 2. To knock - down the expression level of KLF2 in bEnd.3 cells, cells were transfected with Ad-KLF2 siRNA (Genscript, Nanjing, China) and Lipofectamine 3000 (Genscript, Nanjing, China) … WebMar 2, 2024 · BMECs (bEnd.3 or HBMEC) with 3 × 10 7 cells were lysed in 500 μL co-IP buffer (Beyotime Biotechnology), and incubated with 5 μg of mTOR (CST, 2983) or E2F1 … pottery barn furniture knock offs